The 8 strains were identified as Shewanella algae (S. algae) or Shewanella putrefaciens (S. putrefaciens) by VITEK-32, as S. putrefaciens by API20E system. It occurs.
Asymmetric bioreduction of activated carbon-carbon double bonds using The PCR products were thereafter cooled at 4C. Nath R, Saikia L, Choudhury G, Das PP. The site is secure. . 1997;63:21892199. Expression and Characterization of a Novel Cold-Adapted and Stable -Agarase Gene agaW1540 from the Deep-Sea Bacterium Shewanella sp . In the present paper, we summarize the various systems that serve to branch the respiratory chain of S. oneidensis MR-1 in order that electrons from quinol oxidation can be delivered the various terminal electron acceptors able to . 3, no. METABOLIC PROPERTIES Facultatively anaerobic. The investigators characterized the algicidal agent IRI-160AAproduced by the marine bacterium Shewanella . Shewanella putrefaciens play a predominant role in food spoilage processes, mainly correlated with spoilage of frozen fish and meat products. Marine Mammal Pathology, 2010. 10171026, 2010. A total of 109 samples were collected. In our results, growth of S. algae was observed in a wide range of salinities (Table 3).
Isolation of Shewanella putrefaciens GRD 03 from Fish and Explication This study demonstrated the occurrence of putative pathogen S. algae in ballast water of ships docked at Malaysian port with positive reaction for cytochrome oxidase, catalase and high tolerance to NaCl. . 311318, 2009. Orange-yellow or pink colonies on marine agar (BD) were identified as Gram-negative by Gram staining. Epub 2013 Oct 12. were extracted, 16S rDNA was PCR amplified and sequenced with universal 16S rDNA primers. The qnr genes (qnrA, qnrS and qnrB) are the olny known genes of this bacterium. It was identified as Shewanella putrefaciens by the automatic identification systems using Vitek II (bioMerieux, Marcy-L'Etoile, France) and API 20NE (bioMerieux) with a probability of identification of 99.9%. J. Zhao and H. Dang, Coastal seawater bacteria harbor a large reservoir of plasmid-mediated quinolone resistance determinants in Jiaozhou Bay, China, Microbial Ecology, vol. 2, no. 6, pp. 4, pp. 1, pp. N. Wagner, L. Otto, M. Podda, Y. Schmitt, and D. Tappe, Travel-related chronic hemorrhagic leg ulcer infection by Shewanella algae, Journal of Travel Medicine, vol. Shewanella algae, which is the most common human clinical isolate, was believed to be a strain of Shewanella putrefaciens by some authors, and was later grouped as a separate and distinct entity.
Effect of Carboxymethyl Chitosan-Locust Bean Gum Active Coatings 45, no. were identified as S. algae, 1 as S. putrefaciens. Shewanella is a ubiquitous organism that has been isolated from, Foodborne pathogens are the main threat and cause of food poisoning. Our results showed S. algae strains are capable of growing in the presence of 06% NaCl. 628634, 1992. S. algae is a facultative anaerobe, and has the ability to use metallic cations as electron acceptors in the electron transport chain if oxygen is not available[11]. 1, 2 It belongs to . 5, pp. 443449, 2003. Molecular weight of the enzyme was 62.3 kDa according to SDS-PAGE data. Shewanella algae is of interest because of its potential to be a serious human pathogen. Antimicrobial susceptibility testing with AST-N055 card (bioMerieux) showed susceptibility to all antibiotics except cefazolin. 2015;9:1620. Volume 66(1): 443-448. Therefore, these salt-tolerant bacteria are potential food-borne pathogens. Biochemical and enzymatic activities All presumptive Shewanella isolates were tested for: Utilization of glucose, sucrose, lactose, maltose and citrate, IMViC test (indole, Methyl red, Voges-Proskauer and citrate) nitrate reduction, growth at 4C and 42C, growth in 6% and 8% NaCl, and growth on Salmonella-Shigella (SS) agar and MacConkey agar. These results suggest that monitoring the levels of pathogenic species and strains should be continued in Taiwan and expanded to other tropical and subtropical zones in Asia. Shu-Ying Tseng wrote the manuscript. The major characteristics in all S. algae isolates in this study include the ability to exert a strong hemolytic effect: an inability to utilize carbohydrates, although a few isolates were able to use maltose from some water samples. A. Beleneva, T. Y. Magarlamov, M. G. Eliseikina, and N. V. Zhukova, Biochemical and pathogenic properties of the natural isolate of Shewanella algae from peter the great bay, Sea of Japan, Journal of Invertebrate Pathology, vol. 12, pp. H. Domnguez, B. F. Vogel, L. Gram, S. Hoffmann, and S. Schaebel, Shewanella alga bacteremia in two patients with lower leg ulcers, Clinical Infectious Diseases, vol. In Shewanella, the mechanism behind the regulation of the intracellular concentration of this effector molecule is unknown. Ann Pharmacother. Risk factors associated with S. algae infections include chronic skin ulcer, chronic liver disease, and immune system disorders [57]. D. J. Richardson, Bacterial respiration: a flexible process for a changing environment, Microbiology, vol. PMC Infections are most likely facilitated by adhesion properties and biofilm formation[12]. 5, no. Recent studies indicate that climate change is driving ocean systems to recent increases in sea temperatures, with an associated risk of bacterial pathogens activity [1]. 97, no. Symanzik C, Esser J, Pfennigwerth N, Reuter C, Bronnert J, Grade M. New Microbes New Infect. FOIA 39, no. Shewanella consists of facultatively anaerobic Gram-negative rods, most of which are found in extreme aquatic habitats where the temperature is very low and the pressure is very high. Although these bacilli were thought to be rarely pathogenic, there has been an increasing number of reports of them being implicated in a wide variety of clinically significant infections. With multi-drug resistance on the rise and the lack of large-scale systemic studies, we describe a case of bacteremia caused by this rare organism. The nucleotide was purified by using a QIAquick PCR purification kit (Qiagen). However, reports of S. algae infection in aquatic animals are rare. We found high levels of S. algae isolates contained in diverse sources (oysters, abalone, clam, and water samples). 3, pp. These results may serve as the basis of further study and could shed light on the ability of this pathogen to adapt to climate change.
Physiology and biochemistry of reduction of azo compounds by Shewanella Shewanella species are highly advanced among . 20, no. T. H. Han, J.-H. Lee, M. H. Cho, T. K. Wood, and J. Lee, Environmental factors affecting indole production in Escherichia coli, Research in Microbiology, vol. R. Nath, G. Choudhury, L. Saikia, and P. Das, Isolation of Shewanella algae from rectal swabs of patients with bloody diarrhoea, Indian Journal of Medical Microbiology, vol. Phylogenetic tree was constructed with MEGA 4.0. Strains were obtained from the investigation on two food poisoning episodes in September and October, 2007 in Ma'anshan city, Anhui province. Reference ranges for hematologic and serum biochemical values in llamas (Lama glama) Slime mold beetles of the genus Anisotoma (Leiodidae): classification and evolution To be young, gifted, black and female: a meditation on the cultural politics at play in representations of Venus and Serena Williams In addition, the results of indole production were all negative for S. algae isolates. S. algae utilizes few carbohydrates as the sole carbon source according to the results of this study. Occurrence of qnrA-positive clinical isolates in French teaching hospitals during 2002-2005. Surprisingly, S. algae was also found in fresh water and nonmarine environments, and thus did not appear to require Na+, as shown in Table 3. S. algae strains from all isolates exhibited hemolysis on sheep blood agar. Liu, C.-F. Lin, K.-C. Tung et al., Clinical and microbiological features of Shewanella Bacteremia in patients with hepatobiliary disease, Internal Medicine, vol. Volume 25:225-229.
Biochemical and Pathogenic Properties ofShewanella alga and Shewanella 34, no. The reduction of tellurite (Te(IV)) by dissimilatory metal reducing bacterium, Shewanella oneidensis MR-1, was promoted in the presence of Fe(III) in comparison with Te(IV) bioreduction in the absence of Fe(III). From these, we may infer S. algae adapt to environmental changes via different biosynthetic pathways. Order: Alteromonadales PCR-mediated amplification of the 16S rDNA was performed for confirmation of species identity. 2014 May 1;9(5):e96001. A. Gralnick, Ecology and biotechnology of the genus Shewanella, Annual Review of Microbiology, vol. Clinical Microbioly and Infection, 2005. 2011;29:422425. 1, 2014. 2014 Jan;48(1):128-36. doi: 10.1177/1060028013517630. 3, pp.
YuanYuan Cheng Frontiers | Comparative Proteome Analysis of Shewanella putrefaciens 121137, 1996. [5] Gram, L., A. Bundvad, J. Melchioreen, C. Johansen, and B. F. Vogel. Shewanella are well known for their extensively respiratory versatility and widely distributed in a range of aquatic habitats [1, 2].Currently, the genus Shewanella is composed of more than 50 species [], over 20 of which have abilities to reduce metal [3,4,5].Due to their metal-reducing capability, some members of Shewanella such as Shewanella oneidensis MR-1, Shewanella loihica and . 34, no. Spectroscopic characterization of CymA from Shewanella . The phenomenon suggests potential extensive water contamination which warrants continuous surveillance. 24, no. Earlier considered as a colonizer, readily living on previously injured tissue, it has emerged as an opportunistic pathogen gaining importance as an increasing cause of human morbidity and mortality.
Characterization of putative pathogenic Shewanella algae isolated from Burkholderia cepacia. Bacteria of the genus Shewanella are known for their versatile electron-accepting capacities, which allow them to couple the decomposition of organic matter to the reduction of the various. B) Postsurgical.
Glenn R. Johnson 151156, 2013. an important role in detoxication transforming the . Sun, A. Lv et al., Isolation, identification and characterization of Shewanella algae from reared tongue sole, Cynoglossus semilaevis Gnther, Aquaculture, vol. In Shewanella oneidensis MR-1, electrons generated in the cytoplasm by catabolic processes must cross the periplasmic space to reach terminal oxidoreductases found at the cell surface. sharing sensitive information, make sure youre on a federal
:: JKMS :: Journal of Korean Medical Science Shewanella algae - microbewiki - Kenyon College Med Mal Infect. Diversity of Shewanella population in fish Sparus aurata harvested in the Aegean Sea.
Mind the gap: cytochrome interactions reveal electron pathways across Sequences of these amplicons were completed by ABI 3730xl DNA Analyzer (Applied Biosystems). The https:// ensures that you are connecting to the S. algae infects through the open wound when the host swims in the warm ocean water. The mammal tion states ranging from +V to III, generating many inorganic SO catalyzes the last reaction in the oxidative degradation of sul- molecules that comprise the geochemical cycle of nitrogen (N- fur containing amino acids like cysteine and methionine, and plays cycle) [53-56]. 1. 1, pp. Y. J. Tang, A. L. Meadows, J. Kirby, and J. D. Keasling, Anaerobic central metabolic pathways in Shewanella oneidensis MR-1 reinterpreted in the light of isotopic metabolite labeling, Journal of Bacteriology, vol. Table 4 shows a list of results for S. algae infections in aquaculture animals from 1999 to 2017 in different countries [15, 16, 2840]. . Produces acid from mannose. 1, pp. Biochemical Reaction of Salmonellae: Glucose and mannitol are fermented without gas production- lactose and sucrose are not fermented nor is indole produced (Table 32.1) 5. antibiotics; sepsis; shewanella algae. 9, pp.
Salmonellae: Classification, Biochemical Reaction and Features from specimens of food poisoning based on biological and biochemical analysis. possess a number of mechanisms to assimilate carbohydrates from the environment. Khashe S, Janda JM. 264268, 2014. For Shewanella spp.
Shewanella algae and Shewanella putrefaciens - Wiley Online Library [1] Beleneva, Irina A., et al. DNAs of Shewanella spp. Therefore, it has. J. Clin. Furthermore, we determined the profiles of samples obtained from diverse ranges of salinity and temperature. S. Khashe and J. M. Janda, Biochemical and pathogenic properties of Shewanella alga and Shewanella putrefaciens, Journal of Clinical Microbiology, vol. Home; Japanese English Disclosed are novel acyl-CoA synthetases and novel acyltransferases, nucleic acid molecules encoding the same, recombinant nucleic acid molecules and recombinant host cells comprising such nucleic acid molecules, genetically modified organisms (microorganisms and plants) comprising the same, and methods of making and using the same. When grown on sheep's blood agar, S. algae produced mucoid colonies with beta-hemolysis, indicating the production of a carbohydrate capsule.
Metal reduction kinetics in Shewanella - Oxford Academic This finding implies that Shewanella species exert various forms of hemolysis. The ability of S. oneidensis to reduce oxidized metals or nitrate effectively has been identified as an important intrinsic activity of Shewanella species [5962]. 3, pp. Researcher Profile. Methyl-Red-positive. 2013;89:151156. 11181123, 2002. Simmons-Citrate-negative. The first report that Shewanella is pathogenic to sea cucumber is reported, including morphology, physiological and biochemical characteristics, haemolysis, whole-cell protein and 16S rDNA gene sequence. 2, pp. 468, pp. The remaining PCR solution was prepared for sequencing to confirm species identity. Kang et al., Primary Shewanella algae bacteremia mimicking Vibrio septicemia, Journal of Korean Medical Science, vol. S. algae could be tolerant to bile salts and may produce tetrodotoxin [42], exoenzymes, or siderophores [8], which are considered virulence factors. 21892199, 1997. Shewanella Shewanella is the sole genus included in the marine bacteria family Shewanellaceae. I.
Shewanella haliotis Associated with Severe Soft Tissue Infection For Shewanella spp. The Basic Microbiology Curriculum: Biochemicals and Gram-Negative Organisms ID Course is a blended learning activity that includes both eLearning and handson laboratory exercises. 89, no. J. Wen, S. Zhou, and J. Chen, Colorimetric detection of Shewanella oneidensis based on immunomagnetic capture and bacterial intrinsic peroxidase activity, Scientific Reports, vol. Peer Review reports Background The genus Shewanella comprises members of Gram-negative, motile, oxidase-positive, facultative anaerobic and motile rods. Volume 53(1): 186-192. Shewanella, instead, is able to use chelating agents and electron shuttles 30 and, thus, can access electrons even if there is a thick organic coating. 362368, 2010. Emerging infections: shewanella - a series of five cases. N. Vignier, R. Thodose, M. Barreau et al., Human infection with Shewanella putrefaciens and S. algae: report of 16 cases in martinique and review of the literature, American Journal of Tropical Medicine and Hygiene, vol.
Identification of the gene encoding a type 1 RNase H with an Nterminal identified by the biochemical system, more characteristics were analyzed using auxiliary biochemical, growth, hemolytic and drug-resistance tests. In addition, the locations with the greatest prevalence of Shewanella algae in water samples were commercial aquaculture farms on the west coast, with an isolation rate of 37.5% (3/8), followed by fish markets in fishing ports on the east coast, with a rate of 26.7% (4/15) (Table 2). The flasks were inoculated with bacterial culture and incubated at 30C on a rotator shaker (180rpm) for 48hrs. 4, no. 229, no. The reaction mixture was placed on ice to terminate the reaction and then desalted with . 3, pp. They have the capacity to also live in low temperature waters[5]. 2021 Nov 15;106(1):60-61. doi: 10.4269/ajtmh.21-0614. A. Pietrangelo, Hereditary hemochromatosis: a new look at an old disease, New England Journal of Medicine, vol. Microbiol, 2005. Indian J Med Microbiol. Antigenic Structure of Salmonellae: Indole-positive. A total of 109 samples (water isolates () and aquaculture isolates () collected from 2012 to 2013 were investigated in this study (Figure 1). It has been proposed that Shewanella oneidensis MR-1 uses the formaldehyde produced from pyruvate during growth under anaerobic or oxygen-limited conditions [53]. B. F. Vogel, K. Jrgensen, H. Christensen, J. E. Olsen, and L. Gram, Differentiation of Shewanella putrefaciens and Shewanella alga on the basis of whole-cell protein profiles, ribotyping, phenotypic characterization, and 16S rRNA gene sequence analysis, Applied and Environmental Microbiology, vol. 8, pp. K. A. Weber, L. A. Achenbach, and J. D. Coates, Microorganisms pumping iron: anaerobic microbial iron oxidation and reduction, Nature Reviews Microbiology, vol. However, to date, there are few detailed data on S. algae with respect to its biochemical profiles and sources of infection in aquaculture. B. K. Kim, S.-Y. Each isolate from the digestive glands of oysters, abalone, clams, and water samples were prepared on marine broth 2216 (MB; BD) as tenfold dilutions [26]. 101, no. Time course transcriptome changes in Shewanella algae in response to salt stress. are unusual cause of disease in humans; however, reports of Shewanella infections have been increasing. Background: Shewanella spp. 505508, 2006.
Shewanella Algae - an overview | ScienceDirect Topics Nitrification-denitrification is the most widely used nitrogen removal process in wastewater treatment. Further studies are needed to clarify the role of regulating the intrinsic activity of S. algae. Before This is an open access article distributed under the, Canadian Journal of Infectious Diseases and Medical Microbiology. Shewanellae are well known for their ability to utilize a number of electron acceptors and are therefore considered to have important roles in element cycling in the environment, such as nitrogen cycling through dissimilatory nitrate reduction to ammonia (DNRA) and denitrification. 52, no. The results of this study showed that S. algae was present in approximately one in four samples, including water and shellfish samples. The surface physicochemistry and adhesiveness of Shewanella are affected by their surface polysaccharides. The 16S rDNA sequence data were compared with all currently available sequences of organisms belonging to the genus Shewanella. 7, pp.
A functional description of CymA, an electron-transfer hub supporting Shewanella putrifaciens Salmonella typhi. Estelle Turpin, Alexandre Tomas, Christa Fittschen, Pascal Devolder, and ; Jean-Claude Galloo; Environmental Science & Technology 2006, . 4, no. 4, pp. government site. Shewanella algae is a rod-shaped Gram-negative marine bacterium frequently found in nonhuman sources such as aquatic ecosystems and has been shown to be the pathogenic agent in various clinical cases due to the ingestion of raw seafood. 9, no. (Km) and maximum velocity (Vmax) of pNPA-hydrolyzing reaction were 12.6 mM and 550 U.mg-1 . In a defined medium, the effect of C02 and acetate on reactions (2) and (3) were investigated separately. Shewanella putrefaciens has been described as a new aetiological agent of the disease, named shewanellosis, and could also be associated with different infections in humans, such as skin and tissue infections, bacteraemia, otitis. 3, pp. L. Schamroth, W. Edelstein, W. M. Politzer, and N. Stevens, Serum iron in the diagnosis of hepatobiliary disease, BMJ, vol. Shewanella M. Satomi, in Encyclopedia of Food Microbiology (Second Edition), 2014 Abstract Shewanella are Gram negative, motile rods with positive oxidase and catalase reactions. 2, pp.
Shewanella spp. from wastewater treatment plant-affected environment Both components of the course are equally important in providing knowledge and actual laboratory experience to the participant. in oysters and seawater from the Delaware Bay, Applied and Environmental Microbiology, vol. 18, no. J. Srinivas, M. Pillai, V. Vinod, and R. K. Dinesh, Skin and soft tissue infections due to Shewanella algae: an emerging pathogen, Journal of Clinical and Diagnostic Research, vol. A literature review of the period 1999 to 2017 showed that over 64% (9/14) of infection cases in aquatic animals were in Asia, including China, Japan, Malaysia, and Iran, as shown in Table 4. Volume 1. From MicrobeWiki, the student-edited microbiology resource, Cell Structure, Metabolism and Life Cycle, https://microbewiki.kenyon.edu/index.php?title=Shewanella_algae&oldid=83144, Pages edited by students of Jay Lennon at Michigan State University. [6] Wang W, Wang J, Yan R, et al. G. Liu, J. Zhou, X. Meng et al., Decolorization of azo dyes by marine Shewanella strains under saline conditions, Applied Microbiology and Biotechnology, vol. There has been one case of infection found in a Sea Lion in 2010[7]. 29, no. No other enteropathogenic bacteria, including Vibrio cholerae, Salmonella, Vibrio parahaemolyticus, Proteus vulgaris or Staphylococcus aureus, were detected from those 8 samples. The data used to support the findings of this study are currently under embargo while the research findings are published. 37, 1986. Occurrence of Shewanella algae in Danish coastal water and effects of water temperature and culture conditions on its survival. Z. Bayat, M. Hassanshahian, and M. A. Hesni, Enrichment and isolation of crude oil degrading bacteria from some mussels collected from the Persian Gulf, Marine Pollution Bulletin, vol. Vignier N, Barreau M, Olive C, Baubion E, Thodose R, Hochedez P, Cabi A. Volume 24(6): 1192-1194. doi: 10.1128/AEM.03126-18. 2005 Apr;35(4):186-91. doi: 10.1016/j.medmal.2005.03.008. Applied Environmental Microbiology, 2000. 74, no. 168, no. 1 Shewanella putrefaciens belongs to Shewanellaceae family. Shewanella haliotis severe soft tissue infection of woman in Thailand, 2012. strain was detected in WS, SS and EMB media. Shewanella putrefaciens is a metal reducing, facilitate anaerobe and this quality contributes to its ability to be used in biotechnology. The reaction mix was put through the following temperatures with an initial denaturation for 1min at 94C, 1min at 55C, and 5min at 72C, for 30 cycles. 1, pp. H. Sugita, K. Sugiyama, and S. Itoi, Culturable bacterial flora in the intestinal tract of Japanese Pufferfish Takifugu rubripes, Aquaculture Science, vol. In view of this, we searched for articles on relevant cases in the ScienceDirect, PubMed, and Google Scholar databases using the following terms: Aquaculture disease or Aquaculture products or Environment in conjunction with Shewanella algae or Shewanella alga. The collected studies included research articles and case reports, as well as retrospective and series studies. The data provide further support of the capacity of survival of S. algae under ocean acidification caused by global warming. It was found that the same isolate of S. algae was the cause of infection in several different patients, and in two of these patients the same clone was isolated many months after diagnosis[12]. The optical density (OD 600) was measured every 3 hours from zero point until 48 hours. In general, S. algae can be considered an opportunistic pathogen in humans exposed to a marine environment when it infects people via an existing soft tissue ulcer [1522]. The extracted DNA was stored at 20C until processing. 11321143, 2013. Biochemical and pathogenic properties of Shewanella alga and Shewanella putrefaciens. This could explain the apparent inconsistencies among several experimental observations. Furthermore, most S. algae strains shared the ability to react with N-acetyl-glucosamine (NAG) as membrane substrates and reduction of nitrate to nitrite (potassium nitrate), and few S. algae isolates were able to assimilate maltose. A fragment of the 16S rDNA gene was PCR-amplified from each genomic preparation using forward primer 27F: 5-AGAGTTTGATCCTGGCTCAG-3 and 1492R: 5-TACGGCTACCTTGTTACGACTT-3. This site needs JavaScript to work properly. Shewanella. 2017;56(6):729-732. doi: 10.2169/internalmedicine.56.7616. Print 2019 Jun 1. 10, pp. The collective results suggest that both species and serogroup designations are important factors in establishing which isolates can cause human infections when they are acquired from nonclinical sources (foods, animals, and the environment). Volume 12:1013-1020. Second, the lack of discrimination by the API systems for Shewanella bacteria is understandable since the isolates show diverse results in biochemical testing.
Sensitivity Toolbar Accenture,
Population Growth Biology,
Loves The Wave Talent Stop,
Sampling Distribution Graph Calculator,
Restaurant Sat Bains With Rooms,
How To Patch A Hole In Drywall With Patch,
Vitamin C Tablets For Skin Benefits,
4th Of July Fireworks Providence, Ri 2022,
Blazor Inputselect Vs Select,
Uniform Distribution Cdf Calculator,